David Lougheed

I am a software developer at the Canadian Centre for Computational Genomics (C3G) in Montréal, primarily working on creating web portals for health and -omics data.

I have a B.Sc. in Computer Science and Biology (graduated 2019 with First Class Honours), and an M.Sc. in Human Genetics (granted 2023); both from McGill University. My Master's thesis was supervised by Professor Guillaume Bourque, and is titled “Using High-Fidelity Long-Read Sequencing to Better Detect and Understand Short Tandem Repeat Variation in Humans.”

Publications

• EpiVar Browser: advanced exploration of epigenomics data under controlled access (2024)

  David R Lougheed, Hanshi Liu, Katherine A Aracena, Romain Grégoire, Alain Pacis, Tomi Pastinen, Luis B Barreiro, Yann Joly, David Bujold, Guillaume Bourque. Bioinformatics. DOI: 10.1093/bioinformatics/btae136

• Epigenetic variation impacts individual differences in the transcriptional response to influenza infection (2024)

  Katherine A Aracena, Yen-Lung Lin, Kaixuan Luo, Alain Pacis, Saideep Gona, Zepeng Mu, Vania Yotova, Renata Sindeaux, Albena Pramatarova, Marie-Michelle Simon, Xun Chen, Cristian Groza, David Lougheed, Romain Grégoire, David Brownlee, Yang Li, Xin He, David Bujold, Tomi Pastinen, Guillaume Bourque, Luis B Barreiro. Nature Genetics. DOI: 10.1038/s41588-024-01668-z

• How to barcode (almost all) freshwater biodiversity (2023 preprint)

  Orianne Tournayre, Haolun Tian, David R Lougheed, Matthew JS Windle, Sheldon Lambert, Jennipher Carter, Zhengxin Sun, Jeff Ridal, Yuxiang Wang, Brian F Cumming, Shelley E Arnott, Stephen C Lougheed. bioRxiv Preprint. DOI: 10.1101/2023.08.03.551309

• baRcodeR: An open‐source R package for sample labelling (2020)

  Yihan Wu, David R. Lougheed, Stephen C. Lougheed, Kristy Moniz, Virginia K. Walker, Robert I. Colautti. Methods in Ecology and Evolution. DOI: 10.1111/2041-210X.13405

• Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations (2019)

  Janin Grajcarek, Jean Monlong, Yoko Nishinaka-Arai, Michiko Nakamura, Miki Nagai, Shiori Matsuo, David Lougheed, Hidetoshi Sakurai, Megumu K. Saito, Guillaume Bourque & Knut Woltjen. Nature Communications. DOI: 10.1038/s41467-019-12829-8

Software & Projects

• SNIPe

  SNIPe is a web tool for optimally choosing environmental DNA (eDNA) primer pairs to identify a set of taxa. Part of an upcoming publication by Tournayre et al. (currently in preprint; 2023)

• STRkit

  STRkit is a short tandem repeat (STR) genotyper for long read sequencing data such as PacBio HiFi. STRkit was created as part of my Master's degree in Human Genetics at McGill.

• EpiVar Browser

  A browser for exploring quantitative trait loci and their associations with genotype and ancestry. Part of an upcoming publication by Aracena et al. (2024)

• Bento

  A platform for storing, analyzing, and visualizing genomic and epigenomic data, created while working at the Canadian Centre for Computational Genomics (C3G).

• David's Trail Guide Software

  A set of three components for creating mobile trail guide applications: a content server with a corresponding front-end and a white-label Expo application. I used this to build the latest version of the Elbow Lake Trail Guide.

• MHCut Browser (2019)

  A browser for microhomologies at variant CRISPR/Cas9 cut sites. Part of a publication by Grajcarek et al. (2019)

• Elbow Lake Trail Guide

  An interactive trail guide app available on Android and iOS.

• Canadian Field Stations (2014-2016; 2024)

  A compendium of field stations across Canada which I assist in maintaining.